Part:BBa_K2570019:Design

tetR+GFP+mazF+PLtetO1+RFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2590
  Illegal AgeI site found at 2702
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We have added an enzyme cutting site in the design to facilitate BIOBRICK.

anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.

Source

We ordered the part DNA from a synthesis company and had codon optimization of the sequence.

References

1. Soma, Y., Fujiwara, Y., Nakagawa, T., Tsuruno, K., & Hanai, T. (2017). Reconstruction of a metabolic regulatory network in Escherichia coli for purposeful switching from cell growth mode to production mode in direct GABA fermentation from glucose. Metabolic Engineering, 43(August), 54–63.

2. Soma, Y., Tsuruno, K., Wada, M., Yokota, A., Hanai, T., 2014. Metabolic flux redirection from a central metabolic pathway toward a synthetic pathway using a metabolic toggle switch. Metab. Eng. 23, 175–184.